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Image Search Results


Key resources

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Key resources

Article Snippet: Mouse TIMP2 levels were detected in plasma diluted 1:5000 using a mouse TIMP-2 DuoSet ELISA (DY6304, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Recombinant, Diagnostic Assay, Plasmid Preparation, Staining, Extraction, Sterility, Saline, Construct, Electron Microscopy, Expressing, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Multiplex Assay, Transfection, Sequencing, Software, Control

Characterization of TIMP2 protein constructs

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Characterization of TIMP2 protein constructs

Article Snippet: Mouse TIMP2 levels were detected in plasma diluted 1:5000 using a mouse TIMP-2 DuoSet ELISA (DY6304, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Concentration Assay

Description of mouse cohorts

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Description of mouse cohorts

Article Snippet: Mouse TIMP2 levels were detected in plasma diluted 1:5000 using a mouse TIMP-2 DuoSet ELISA (DY6304, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques:

Hippocampal gene expression following treatment with TIMP2 and TIMP2-hIgG4

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Hippocampal gene expression following treatment with TIMP2 and TIMP2-hIgG4

Article Snippet: Mouse TIMP2 levels were detected in plasma diluted 1:5000 using a mouse TIMP-2 DuoSet ELISA (DY6304, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Expressing

Few significant changes in hippocampal gene expression following treatment with TIMP2 or TIMP2-hIgG4

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Few significant changes in hippocampal gene expression following treatment with TIMP2 or TIMP2-hIgG4

Article Snippet: Mouse TIMP2 levels were detected in plasma diluted 1:5000 using a mouse TIMP-2 DuoSet ELISA (DY6304, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Expressing

The TIMP2 constructs had distinct MMP inhibitory profiles

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: The TIMP2 constructs had distinct MMP inhibitory profiles

Article Snippet: Mouse TIMP2 levels were detected in plasma diluted 1:5000 using a mouse TIMP-2 DuoSet ELISA (DY6304, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Construct, Inhibition

The alanine insertion into TIMP2 prevented MMP inhibitory activity at biologically relevant concentrations

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: The alanine insertion into TIMP2 prevented MMP inhibitory activity at biologically relevant concentrations

Article Snippet: Mouse TIMP2 levels were detected in plasma diluted 1:5000 using a mouse TIMP-2 DuoSet ELISA (DY6304, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Activity Assay

MMP protein constructs. Schematic depicting the nine MMP protein constructs assessed for binding to TIMP2 constructs using bio-layer interferometry (BLI) and surface plasmon resonance (SPR). CD, catalytic domain.

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: MMP protein constructs. Schematic depicting the nine MMP protein constructs assessed for binding to TIMP2 constructs using bio-layer interferometry (BLI) and surface plasmon resonance (SPR). CD, catalytic domain.

Article Snippet: Mouse TIMP2 levels were detected in plasma diluted 1:5000 using a mouse TIMP-2 DuoSet ELISA (DY6304, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Construct, Binding Assay, SPR Assay

Characterization of TIMP2-MMP binding of the TIMP2 constructs

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Characterization of TIMP2-MMP binding of the TIMP2 constructs

Article Snippet: Mouse TIMP2 levels were detected in plasma diluted 1:5000 using a mouse TIMP-2 DuoSet ELISA (DY6304, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Binding Assay

Key resources

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Key resources

Article Snippet: Human TIMP2 levels in the plasma and brain were detected using a human TIMP-2 DuoSet ELISA (DY971, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Recombinant, Diagnostic Assay, Plasmid Preparation, Staining, Extraction, Sterility, Saline, Construct, Electron Microscopy, Expressing, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Multiplex Assay, Transfection, Sequencing, Software, Control

Characterization of TIMP2 protein constructs

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Characterization of TIMP2 protein constructs

Article Snippet: Human TIMP2 levels in the plasma and brain were detected using a human TIMP-2 DuoSet ELISA (DY971, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Concentration Assay

Description of mouse cohorts

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Description of mouse cohorts

Article Snippet: Human TIMP2 levels in the plasma and brain were detected using a human TIMP-2 DuoSet ELISA (DY971, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques:

Hippocampal gene expression following treatment with TIMP2 and TIMP2-hIgG4

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Hippocampal gene expression following treatment with TIMP2 and TIMP2-hIgG4

Article Snippet: Human TIMP2 levels in the plasma and brain were detected using a human TIMP-2 DuoSet ELISA (DY971, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Expressing

Few significant changes in hippocampal gene expression following treatment with TIMP2 or TIMP2-hIgG4

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Few significant changes in hippocampal gene expression following treatment with TIMP2 or TIMP2-hIgG4

Article Snippet: Human TIMP2 levels in the plasma and brain were detected using a human TIMP-2 DuoSet ELISA (DY971, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Expressing

The TIMP2 constructs had distinct MMP inhibitory profiles

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: The TIMP2 constructs had distinct MMP inhibitory profiles

Article Snippet: Human TIMP2 levels in the plasma and brain were detected using a human TIMP-2 DuoSet ELISA (DY971, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Construct, Inhibition

The alanine insertion into TIMP2 prevented MMP inhibitory activity at biologically relevant concentrations

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: The alanine insertion into TIMP2 prevented MMP inhibitory activity at biologically relevant concentrations

Article Snippet: Human TIMP2 levels in the plasma and brain were detected using a human TIMP-2 DuoSet ELISA (DY971, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Activity Assay

MMP protein constructs. Schematic depicting the nine MMP protein constructs assessed for binding to TIMP2 constructs using bio-layer interferometry (BLI) and surface plasmon resonance (SPR). CD, catalytic domain.

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: MMP protein constructs. Schematic depicting the nine MMP protein constructs assessed for binding to TIMP2 constructs using bio-layer interferometry (BLI) and surface plasmon resonance (SPR). CD, catalytic domain.

Article Snippet: Human TIMP2 levels in the plasma and brain were detected using a human TIMP-2 DuoSet ELISA (DY971, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Construct, Binding Assay, SPR Assay

Characterization of TIMP2-MMP binding of the TIMP2 constructs

Journal: eNeuro

Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

doi: 10.1523/ENEURO.0031-23.2023

Figure Lengend Snippet: Characterization of TIMP2-MMP binding of the TIMP2 constructs

Article Snippet: Human TIMP2 levels in the plasma and brain were detected using a human TIMP-2 DuoSet ELISA (DY971, R&D Systems) and DuoSet Ancillary Reagent Kit 2 (DY008, R&D Systems).

Techniques: Binding Assay